Etude Du Mécanisme de La Biotine Synthase - Grenoble, France - Université Grenoble Alpes

Université Grenoble Alpes

Entreprise vérifiée

Grenoble, France

il y a 3 semaines

Posté par:

Sophie Dupont

beBee Recruiter

Description

Etude du mécanisme de la biotine synthase // Investigating the mechanism of biotin synthase:

Réf
ABG-121167

ADUM-54995

Sujet de Thèse 13/03/2024
Université Grenoble Alpes
Lieu de travail
Grenoble cedex
France
Intitulé du sujet
Etude du mécanisme de la biotine synthase // Investigating the mechanism of biotin synthase
Mots clés
Métalloprotéine, Chimie radicalaire, Structurefonction, chimie du soufre, Centre fersoufre
Metalloprotein, Radical chemistry, Structurefunction, Sulfur chemistry, ironsulfur clusterBiotin synthase is the enzyme responsible for the final step in biotin biosynthesis. This enzyme is a member of the radical SadenosylLmethionine (SAM) protein superfamily and uses radical chemistry to catalyze the twostep insertion of a sulfur atom into the thiolane ring.

We recently identified a new type
Description du sujet:
Biotin synthase is the enzyme responsible for the final step in biotin biosynthesis. This
- enzyme is a member of the radical S-adenosyl

L methionine (SAM) protein superfamily and uses

- radical chemistry to catalyze the two-step insertion of a sulfur atom into the thiolane ring. We
- recently identified a new type of biotin synthase that does not sacrifice its auxiliary FeS cluster to
- provide the sulfur atom. In this project, we aim at studying this new enzyme, combining structural
- and biochemical studies with spectroscopic analyses and theoretical calculations to unravel the
- mechanism of this enzyme along two main axes. The first one corresponds to the search for the
- sulfur provider and to the structural and functional characterization of its transfer between the two
- partners. The second axis corresponds to the tracking and characterization of biotin synthase
- reaction intermediates. This project is therefore at the interface between biochemistry, bioinorganic- charge of the protein production, functional analyses, including the search for the physiological
- partner, as well as the different structural characterizations using X-ray crystallography and cryoelectron
- microscopy. They will also participate, through collaboration to the spectroscopic- at the Institut de biologie Structural, which is fully equipped with state-of-the-art gloveboxes to
- work under anaerobic conditions "from gene to structure". They will also work at the Laboratoire
- de Chimie et Biologie des Métaux notably to perform all the in vitro functional analyses and sample- environment to afford a future career either in academia or in R&D.

Début de la thèse : 01/10/2024
Nature du financement:

Précisions sur le financement: