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Postdoc position in Marseille - Biochemical and mechanical guidance of migration in single cells... (BB-C8DFA)

Trouvé dans: Talent FR

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Host laboratory and collaborators / LAI / / IBDM - CPT - LAI / KeywordsMigration, guidance, single cells, cell aggregates, cell and tissue mechanics, gradients, microfluidics, live imagingObjectivesThe objectives will be to (i) characterize the migration of single cells versus external guiding cues, either mechanical (adhesion patterns, confinement) and biochemical (gradient of chemokines) (ii) adapt the microfluidic system to 3D cells aggregates (few hundreds of microns size) and optimize substrate characteristics for their migration (iii) image in 2D and 3D the collective dynamics of cells within the aggregate during the migration and analyze their organization and dynamics in response to external perturbations.Expected profileThe candidate should be interested in biophysics and quantitative reductionist approaches in vitro. An experience in microscopy/image analysis and/or microfluidics and/or cell biology is valuable.2 to 5 references related to the project Beaune, G., C. Blanch-Mercader, S. Douezan, J. Dumond, D. Gonzalez-Rodriguez, D. Cuvelier, T. Ondarçuhu, P. Sens, S. Dufour, Murrell, and F. Brochard-Wyart. 2018. Spontaneous migration of cellular aggregates from giant keratocytes to running spheroids. Proc. Natl. Acad. Sci. 115:12926–12931.Mohammed, D., G. Charras, E. Vercruysse, M. Versaevel, J. Lantoine, L. Alaimo, C. Bruyère, M. Luciano, K. Glinel, G. Delhaye, O. Théodoly, and S. Gabriele. 2019. Substrate area confinement is a key determinant of cell velocity in collective migration. Nat. Phys. 15:858–866.Aoun L, Nègre P, Gonsales C, Seveau de Noray V, Brustlein S, Biarnes-Pelicot M, Valignat M-P and Theodoly O*. 2021. Leukocyte transmigration efficiency and longitudinal forward-thrusting force in a microfluidic Transwell device. 3 main publications from each PI over the last 5 yearsOlivier Theodoly Luo, X., de Noray, L. Aoun, M. Biarnes-Pelicot, P.-O. Strale, V. Studer, M.-P. Valignat, and O. Theodoly. 2020. Lymphocytes perform reverse adhesive haptotaxis mediated by LFA-1 integrins. J. Cell Sci. 133.Aoun, L., A. Farutin, N. Garcia-Seyda, P. Nègre, Rizvi, S. Tlili, S. Song, X. Luo, M. Biarnes-Pelicot, R. Galland, J.-B. Sibarita, A. Michelot, C. Hivroz, S. Rafai, M.-P. Valignat, C. Misbah, and O. Theodoly. 2020. Amoeboid Swimming Is Propelled by Molecular Paddling in Lymphocytes. Biophys. J. 119:1157–1177.Garcia-Seyda, N., L. Aoun, V. Tishkova, V. Seveau, M. Biarnes-Pelicot, M. Bajénoff, M.-P. Valignat, and O. Theodoly. 2020. Microfluidic device to study flow-free chemotaxis of swimming cells. Lab. Chip. 20:1639–1647 Sham Tlili Tlili et al., “Migrating Epithelial Monolayer Flows like a Maxwell Viscoelastic Liquid,” Phys. Rev. Lett. 2020.Tlili et al., Collective cell migration without proliferation: density determines cell velocity and wave velocity R Soc Open Sci. 2018Gauquelin E, Tlili S, Gay C, Peyret G, Mège RM, Fardin MA, Ladoux B. Influence of proliferation on the motions of epithelial monolayers invading adherent strips. Soft Matter. 2019 Apr 7;15(13):2798-2810.

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