Preparation of Nanoemulsions Encapsulating - Villeurbanne, France - Université Lyon 1

Description

Preparation of nanoemulsions encapsulating sensitive biomacromolecules by premix membrane emulsification:

• Réf

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ABG-111284

• Sujet de Thèse 20/02/2023
• Contrat doctoral
• Université Lyon 1
• Lieu de travail
• Villeurbanne
• Auvergne-RhôneAlpes
• France
• Intitulé du sujet
• Preparation of nanoemulsions encapsulating sensitive biomacromolecules by premix membrane emulsification
• Champs scientifiques
• Génie des procédés
• Chimie

• Mots clés

• Membrane emulsification, formulation, sensitive biomacromolecules
  Description du sujet:

The PhD thesis will investigate premix membrane emulsification [2, 3] as a new, gentle and scalable approach for the production of lipid nanoemulsions (LNE) encapsulating sensitive biomacromolecules like peptide and RNA.

The expected benefits are to decrease (or even avoid) the use of solvents, to obtain more concentrated dispersions and to increase the stability of the biomolecules.

In premix membrane emulsification, a coarse emulsion is first prepared, then this emulsion is passed through a porous membrane to homogenize the droplets.

The technique uses low pressures and shear stresses which is advantageous for the processing of biomacromolecules.

The PhD thesis will be divided in different tasks.

Task 1 will investigate the influence of the formulation (choice of lipids and additives, influence of biomolecule structure, etc) on the characteristics of the LNE obtained.

Task 2 will focus on the membrane emulsification technique (influence of the membrane, operating conditions, etc).

The LNE will be characterized by various complementary technics to determine their size, charge, encapsulation efficiency,The stability and integrity of encapsulated biomolecules will also be investigated.

Depending on the results, biocompatibility of the formulations may finally be investigated via _in vitro_ assays.


References
[1] Brader M.L., Williams S.J., Banks J.M., Hui W.H., Zhou Z.H., Jin L. Encapsulation state of messenger RNA inside lipid nanoparticles. Biophysical Journal,

[2] Alliod O., J.P. Valour, S. Urbaniak, H. Fessi, D. Dupin, C. Charcosset, Preparation of oil-in-water nanoemulsions at large-scale using premix membrane emulsification and Shirasu Porous Glass (SPG) membranes, Colloids and Surfaces A,

[3] Nazir A., G.T. Vladisavljević, Droplet breakup mechanisms in premix membrane emulsification and related microfluidic channels, Advances in Colloid and Interface Science,


Prise de fonction:

• 02/10/2023
  Nature du financement:
• Contrat doctoral

Précisions sur le financement:

• Ministerial scholarship, pre-selection and audition in June 2023
  Présentation établissement et labo d'accueil:
• Université Lyon 1

The host laboratory is Laboratoire d'Automatique, de Génie des Procédés et de Génie Pharmaceutique, Laboratory of Automatic Control, Chemical and Pharmaceutical Engineering (LAGEPP), University Claude Bernard Lyon 1.


Site web:


Intitulé du doctorat:

• Dcotorat de Chimie
  Pays d'obtention du doctorat:
• France

Etablissement délivrant le doctorat:

• Ecole doctorale de Chimie de Lyon 20/02/2023