Criblage de Perte de Fonction à L'échelle Du - Marseille e, France - Aix Marseille Université

Aix Marseille Université

Entreprise vérifiée

Marseille e, France

il y a 2 semaines

Posté par:

Sophie Dupont

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Description

Criblage de perte de fonction à l'échelle du génome de Mimivirus // Loss-of-function genome-wide screening of Mimivirus:

Réf
ABG-121588

ADUM-54591

Sujet de Thèse 22/03/2024
Contrat doctoral
Aix Marseille Université
Lieu de travail
MARSEILLE CEDEX 0
France
Intitulé du sujet
Criblage de perte de fonction à l'échelle du génome de Mimivirus // Lossoffunction genomewide screening of Mimivirus
Mots clés
Virus géants, Manipulation Génétique, Génétique avancée, Gènes Orphelins, Mimivirus, Criblage à l'échelle du génome
Giant virus, Genetic manipulation, Forward genetics, ORFan genes, Mimivirus, Genomewide screening
Description du sujet:

Giant viruses (GVs) encode thousands of protein-coding genes. 70% of such genes encode proteins unseen in any other organisms and thus, are ORFan genes. Such genes likely evolved to hijack cellular functions and outcompete other Acanthamoeba pathogens. Concordantly, their potential use for therapeutics, bioengineering, or methodological advances is tremendous.

During the past two years, we have introduced a battery of tools for the manipulation of the genome of giant viruses.

We now aim to utilize those tools to generate a genome-wide platform of knock-out viruses to assess the function of the approx.

1000 genes encoded by mimivirus.

For this PhD project, the applicant will generate a platform of viruses containing a knockout for each gene of mimivirus and use them to address each gene's overall fitness-conferring phenotype and investigate gene function at different stages in the infectious cycle of mimivirus.

In addition, we will use this platform to identify genes associated with competition between pathogenic organisms by forward genetics. Forward genetic screens will be performed in the presence of competitors like pandoravirus (viral) or Babella spp. (bacteria); or infectious agents like the sputnik virophage, which inhibits mimivirus replication.

Overall, the project presented here aims to provide broad-based functional information on mimivirus genes and facilitate future approaches to utilize viral genes for biotechnological purposes.

Début de la thèse : 01/10/2024-
Nature du financement:

Contrat doctoral
Précisions sur le financement:
Concours pour un contrat doctoral
Présentation établissement et labo d'accueil:
Aix Marseille Université

Etablissement délivrant le doctorat: