- Generate stable mammalian cell lines expressing therapeutic proteins of interest
- Optimise expression of genes of interest in mammalian cells by testing different promoters, UTR's, etc.
- Purify recombinant proteins produced in mammalian cells and prepare high-quality samples for downstream analysis
- Characterize purified therapeutic proteins:
- Quantification
- Western blotting
- Activity assays
- Stability assays
- Protein secondary structure determination
- Glycoprofiling using mass spectrometry (sample preparation, execution, and analysis)
- Be an active part of a fast-growing R&D team
- Postdoctoral studies
- Experience in a biotech/pharmaceutical company
- Prior expertise with CHO or HEK cell lines
- Patent inventor
- Experience working with plant and yeast recombinant expression systems
- Strong bioinformatic skills
- French language
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Description
Description poste
A lab-based position, your work will focus on the engineering of mammalian cell lines and analysis of therapeutic proteins produced in engineered cell lines.
Roles and responsibilities
Description profil
PhD in glycobiology, cell and molecular biology, biochemistry, biological engineering or related field
Experience working with glycoproteins and/or the protein secretory pathway
Excellent basic molecular biology techniques: mammalian cell culture, transfections, bacterial cloning, PCR and qPCR, fluorescence microscopy and protein analysis
At least one first author academic paper or equivalent proof of scientific leadership
Mass spectrometry experience
Someone who wants to push the boundaries of scientific knowledge and tackle things they have never done before (or no one has)
Fluency in English
A team player who is strongly invested in our mission at and willing to go the extra mile
Bonus skills:
Poste proposé
Intitulé du poste proposé
– Research Associate – Glycobiology Specialist
Poste basé à Paris
Secteur d'activité : Biotechnology
Nature du contrat : CDI
Disponibilité : A partir de août 2023
