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Soisy-sur-Seine

    Laboratory Technician/Manager - Soisy-sur-Seine, France - Kyron

    Kyron
    Kyron Soisy-sur-Seine, France

    il y a 1 semaine

    Default job background
    CDI
    Description

    Description poste

    Position: Laboratory Technician/Manager
    We are looking for a highly experienced, independent, and capable laboratory technician/manager to kick off experiments in our brand-new lab space in central Paris.
    Roles and responsibilities:

  • Cloning (plasmid design, PCR, E. coli culturing and transformation)
  • Maintenance of mammalian cell lines including mycoplasma testing, propagation,
    and cryopreservation
  • Mammalian cell transfections
  • Use aseptic techniques including working within a biological safety cabinet
  • Purification of recombinant proteins produced in mammalian cells and preparation
    of samples for mass spectrometry
  • Analysis of proteins produced by mammalian cell lines including SDS-PAGE,
    immunoblotting, and immunofluorescence
  • Contribute towards the setup of the company's lab space in central Paris
  • Coordinate the daily running of the laboratory including ordering and maintenance of equipment
  • Description profil

    Requirements:

  • Masters (or equivalent) qualification
  • Minimum 2 years hands-on experience in the laboratory
  • Extensive experience with cloning, PCR amplification, bacterial transformations, and plasmid manipulation
  • Experience with protein analysis (Western blotting & protein purification)
  • Experience with mammalian cell culture and aseptic technique
  • Exceptional organisation skills with strong attention to detail
  • Knowledge of laboratory safety rules
  • Ability to work in a team environment
  • Excellent communication skills
  • Someone who is resourceful and will do whatever it takes to get the job done – Fluent in English and French
  • Bonus skills:

    – Cultured CHO & HEK cell lines
    – Experience working with yeast cells
    – Experience in lab space set up
    – Relevant industrial experience

    Poste proposé

    Intitulé du poste proposé
    – Laboratory Technician/Manager

    Poste basé à Paris

    Secteur d'activité : Biotechnology

    Nature du contrat : CDI

    Disponibilité : A partir de mars 2023


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