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    Ingénieur d'études R&D - Paris, France - L'institut Imagine

    L'institut Imagine
    L'institut Imagine Paris, France

    il y a 5 jours

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    Description

    Créé à l'initiative des chercheurs et médecins de l'Hôpital Necker – Enfants malades, l'Institut hospitalo-universitaire (IHU) sur les maladies génétiques Imagine développe des programmes scientifiques fondés sur une continuité entre recherche fondamentale, recherche clinique et soins innovants sur les maladies génétiques et les prédispositions génétiques aux maladies fréquentes, de l'enfance à l'âge adulte. Imagine a pour ambition d'accélérer la recherche en l'organisant au plus près du malade afin d'apporter les solutions diagnostiques et thérapeutiques attendues par les patients et leurs familles.

    Le projet « Cil primaire et malformations cérébrales » vise à comprendre les mécanismes moléculaires et cellulaires sous-tendant les malformations du cortex cérébral de patients atteints de ciliopathies, en tirant profit de méthodes innovantes basées sur l'utilisation de cellules souches pluripotentes induites (iPS) de patients puis génération de modèles cellulaires 2D et 3D de développement du néocortex rosettes neurales et organoides cérébraux.

    Les principales missions sont :

  • Effectuer l'entretien des cellules iPS
  • Edition du génome des iPS de patients par CRISPR/CAS9 pour générer des contrôles isogéniques
  • Différencier les cellules iPS contrôles et de patients en progéniteurs neuronaux, en organoïdes cérébraux et en différents types de neurones corticaux
  • Caractériser le phénotype de ces différents produits de différenciation
  • Des astreintes de week-end sont éventuellement à prévoir en fonction des besoins

    COMPETENCES

  • Techniques de biologie cellulaire : culture cellulaire (iPS)
  • Techniques de biologie moléculaire : Extraction d'ADN, ARN et protéines, RT-PCR, Western blot
  • Cryostat
  • Immunofluorescence sur cellules et tissus
  • Microscopie confocale
  • CDD d'un an renouvelable

    Salaire selon profil et expérience

    Prise de fonction: dès que possible


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